Medical Technology

How to Perform the Venipuncture Procedure Venipuncture Procedure Venipuncture

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The venipuncture or phlebotomy procedure is one method of blood collection that allows multi-collection with a larger volume of blood.  You can do it with the use of a syringe or a vacutainer whichever you find easier. Not anyone can perform a venipuncture because of some complications that can occur in the process.  Skilled phlebotomists or medical technologists usually perform the procedure.

Materials needed:

Sterile and dry syringe

Sterile dry cotton

Sterile wet cotton

Test tubes


Test tube rack

Labeling tape

Pentel pen or labeling pen



Step 1

Prepare your materials by arranging them in an area within your arm’s reach. Check that the needle and syringe are working well, by pulling and pushing the plunger and examining the needle. Do not remove the cap of the needle unnecessarily.

Step 2

Select the most appropriate site of puncture. A vein that is not too small or too big is ideal. Big veins have the propensity to roll and small veins are less likely visible.  Take time in your selection process. The most common site of puncture is the antecubital fossa at the bend of the arm.  There are three veins you could select from, the cephalic, basilica and mid-cubital veins.

Step 3

When you have selected the best vein, sterilize it in a circular motion, starting from the site of puncture going outwards.  Do not reuse dirty cotton. Sterilize until clean.

Step 4

Let it dry for a few seconds and with your syringe and needle, puncture the site smoothly and deliberately.  There should be no hesitation, as this would inflict more pain to the patient. Enter the vein in one smooth movement.

Step 5

As blood enters the hub of the needle, pull the plunger smoothly taking care not to pull the needle out of the vein.

Step 6

After you have drawn the required amount of blood, withdraw the needle smoothly, not too fast, nor too slow to avoid severing the vein. 

Step 7

Apply pressure to the wound for about 3-5 minutes.  You could allow the patient to do it, if he cannot, then apply micropore.  Be sure to check the wound after 5 minutes to make certain that there is no bleeding.

Step 8

Remove the needle from the syringe and transfer the blood to a test tube by allowing the blood to flow at the sides of the tube to avoid hemolysis. Be sure that your test tube is also dry and clean to avoid hemolysis and contamination. Cover the tube and label it properly.

Step 9

Dispose your used materials properly into appropriate trash bags or cans. Clean your working area properly,

Step 10

Check the wound of the patient and thank him/her for cooperating.


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